Enzyme Function:beta-glucosidase
Accession number: AN0712.2
GenBank ID: DQ490467
FGSC number: 10063
Average molecular weight
(clone, calculated):
95.088 kDa
AA length (clone): 873 aa
DNA length (clone): 2622 bp
DNA source: xylan cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGAGCTTCCAAAAGGTCGATCCTGCGCAGAT AGAGAGCGTGCTCTCTAAGCTGACTCTGGAAGAGAAGATCTCCCTGCTGG CGGGGAAGAACTTCTGGGAAACACAAGACTATCCAGAAAAGGGGGTGCCA CCTGTCAAGACTTCAGATGGTCCCAACGGGGCGCGCGGTGCCACATTCAA GGGAGGAGTAACAGCTGCCTGTTTCCCAGCGTCCTCTCTCTTAGCTGCGA CGTGGGATCTCGACGCGGCAAAGCACATTGGAGAAGCATTGGCTGACGAG ACGCGCTCGAAAGGCGCAAGGGTTCTATTAGCACCCACAGTCTGCATTCA TCGCCATCCTCTGGGAGGCCGCAACTTCGAATCGTTCAGTGAAGATCCTT TCCTTGCAGGAAAGCTCGCGGCGCAGTATATTAAAGGCCTTCAGGGCAAC GGCGTCGCTGCAACCATCAAGCACTATGCCGCGAACGAGCAAGAGACCTG CCGATTCACAGTCAACGAACATATCACAGAACGAGCACTCCGAGAAATCT ACCTGAAACCATTCGAGATCGCCATCAAAGAGTCGAACCCGCTGGCAGTT ATGACCGCCTACAACATTGTGAATGGAACCCACGCTGACTCGAACAATTT TCTCTTGCGAGATGTGCTCAGGGGAGAATGGGGATGGAAGGGTCTTGTTA TGAGTGATTGGGGCGGTACCAACTCCACGGCCGACGCTCTGAATGCTGGT CTTGACCTAGAAATGCCCGGTCCTACAAGGTGGCGCAAGGTAGACGAAGT GCTTGCAGTCGTAAAGTCTGGTGCCGTGTTGGAGGAGACTATCGACGAGC GCGCGCGCAACGTTCTGGAGCTTCTTGCTAAGCTGAATTGTTTCGAAAAT CCTACTATTCCAGAAGAGAAGGCCATCAATCGTCCTGAACACCAGAAATT AATCCGCTCCGTGGGAAGTCAAGGCCTCGTCCTCCTCAAAAACGAGGGCG ATGTTCTTCCTCTGAGAAAAGAGATATTGACCAACAAGAAGGTAGCTCTT CTGGGCTTTGCGCGAGAAGCTCTCATTCACGGCGGTGGCAGTGCTTCAGT CAATGCACATTATCGAGTCACTCCAGAAGAGGGTCTCCGCGCCGCTTTAG GCGACACTGTTGAGTTTGAATATGCCAAGGGTGCACACACTTTCCGCCAG CTGCCCTTAATGAGCGACAACGTTGTTAACCTAGAAGGCCAACCTGGTTG GACCCTAGACTTTTTTGCGGACGAGGAGCCCAACGGCGAGCCAGGCTCAT CGATCTCGTCCGAGCAACCCAGCTATATACCGCTCTTTGTCAAGGAATCA TGGGGAAGTGTCCGAGCCAGCGCCCATTTCACACCCACGCAAAGCGGGAA ACACTATTTTGGCATGTCTGGGTTGGGCCGGTCCAAACTCTTGATCGACG GCGAGGTCATCTACGAACAGAAGCTGAATTGTCCGGACTCCATGGGATTC TTACTCGGCGGCGTGGAAGAGCCAGAAATCCAGTATTCATTCGAAGCTGG AAAGACCTATGCGGTCGAAGTGGTTTCGGTGAAACCCACCAGCAAGGGCG GTCTCGCTCTGCTGGACGGCTTCATTGGTTTCCGTCTCGGGTTCATGACC GAGGAAGAGCACAATAGAGATTTGCTGTCTGAAGCCGTCGATGTAGCCAA ACGTAGTGATATCGCAATTGTCTTCACTGGCCATACTCCAGATTGGGAGA CGGAAGGGCAGGACCAGATAAGCTTCCACCTACCGTCCAACGGTTCCCAG GACCGGCTCGTGGCAGCTGTTGGAGCCGCCAACCCTAACACCGTTGTCGT GAACTGCACTGGCGTAGCGGTTGCTATGCCTTGGTTGGACAAGGTCAAGG CGGTTGTTCAAGCGTGGTTCCCAGGACAGGAAGCCGGCAATGCAATTGCT GACGTACTCACCGGCGCTGTGAACCCTTCTGGACGTCTACCTGTGTCCTT CCCTCGGGCGATCGAGGATGCCCCTGCACACGGTAACTTCCCCGGTGACT ACACAGACGGGAAGGACAACCGGCGCCATCTCGAAGTTACATACAAGGAA GGTGTCTTCGTGGGCTACCGGCACTATGATCTCTCTGAAGCCAACCGCGC CAAGGTCCTGTTCCCGTTCGGCTACGGTCTATCCTACACCACTTTTACAC ATGCGAATCACAAAGCAAGTGCTACGAGCAGGAACACCGTCGAAGTGGCC GTGGATGTCACTAACGTCGGCACCTGTGCTGGAGCTGATGTTGTTCAAGT CTACGCTGGTGCAAAACTGGCAGTCCCAGAGAACCCAGTCAAAGAGCTAG TTGGTTTCGCCAAGGTACACCTCAAGCCGGGGGAGACCAAGACTGCCAAT ATCACATTCGAAGTGCGCCAACTGACTCACTTCACGGAGAGGAGCGGCAA GTGGGAGCTGGAATCAGGCGACTATGAAATATCAATCGGTCAGTCCGTGA GGGATATCACTGGCAAGGTGGAGATTGGTCTGGAAGCCCAGAATTATAAA CCTCTAGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGA CCATCATCATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMSFQKVDPAQIESVLSKLTLEEKISLLAGKNFWETQDYPEKGVP PVKTSDGPNGARGATFKGGVTAACFPASSLLAATWDLDAAKHIGEALADE TRSKGARVLLAPTVCIHRHPLGGRNFESFSEDPFLAGKLAAQYIKGLQGN GVAATIKHYAANEQETCRFTVNEHITERALREIYLKPFEIAIKESNPLAV MTAYNIVNGTHADSNNFLLRDVLRGEWGWKGLVMSDWGGTNSTADALNAG LDLEMPGPTRWRKVDEVLAVVKSGAVLEETIDERARNVLELLAKLNCFEN PTIPEEKAINRPEHQKLIRSVGSQGLVLLKNEGDVLPLRKEILTNKKVAL LGFAREALIHGGGSASVNAHYRVTPEEGLRAALGDTVEFEYAKGAHTFRQ LPLMSDNVVNLEGQPGWTLDFFADEEPNGEPGSSISSEQPSYIPLFVKES WGSVRASAHFTPTQSGKHYFGMSGLGRSKLLIDGEVIYEQKLNCPDSMGF LLGGVEEPEIQYSFEAGKTYAVEVVSVKPTSKGGLALLDGFIGFRLGFMT EEEHNRDLLSEAVDVAKRSDIAIVFTGHTPDWETEGQDQISFHLPSNGSQ DRLVAAVGAANPNTVVVNCTGVAVAMPWLDKVKAVVQAWFPGQEAGNAIA DVLTGAVNPSGRLPVSFPRAIEDAPAHGNFPGDYTDGKDNRRHLEVTYKE GVFVGYRHYDLSEANRAKVLFPFGYGLSYTTFTHANHKASATSRNTVEVA VDVTNVGTCAGADVVQVYAGAKLAVPENPVKELVGFAKVHLKPGETKTAN ITFEVRQLTHFTERSGKWELESGDYEISIGQSVRDITGKVEIGLEAQNYK PLEQKLISEEDLNSAVDHHHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGAGCTTCCAAAAGGTCG (Pml I) TACAGTCTAGAGGTTTATAATTCTGGGCTTCC (Xba I)
pH optimum: 5.5
Specific activity (Substrate): PNP-beta-glucoside
T optimum: 52
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: active on PNP-beta-glucoside
References: not yet available