| Enzyme Function: | pectate lyase |
| Accession number: | AN0741.2 |
| GenBank ID: | DQ490468 |
| FGSC number: | 10064 |
| Average molecular weight (clone, calculated): | 37.913 kDa |
| AA length (clone): | 354 aa |
| DNA length (clone): | 1065 bp |
| DNA source: | xylan cDNA |
| DNA Sequence (clone): | TCGATGAATTCACGTGATATGAGGTTCACCCCTCTCTTCCTGCTGGCCGC TGTGGCCATTGCCTCCCCTGCGCCGGACCTCAACGCCCGTCATGAATTGA CCCGCCGCCAGGCCTCAGAAAGCTGCCCGATCGGGTACTGCACACAGAAC GGTGGCACTACCGGTGGTGCGGCCGGTGACACCGTGACCGTGACCAATCT GGCCGACCTGACTGAAGCCGCCGAGAGCGATGGGCCGCTGACGATCATCG TGTCTGGGTCCATCTCGGGCAGTGCCAAGATCCGCGTGGCCTCAGATAAG ACGATCTTTGGAGAGTCGGGTAGTTCTATCACCGGTATCGGATTCTACAT TCGCCGCGTCAGCAATGTCATCATGCGGAACTTGAAGATCAGCAAGGTCG ACGCAGACAACGGCGATGCCATTGGCATTGATGCCTCCTCCAATGTCTGG GTCGATCATTGCGACCTCTCTGGAGACCTCAGCGGTGGGAAGGATGACTT GGACGGACTGGTCGATATCAGCCATGGCGCGGAATGGATCACCGTCTCGA ACACTTACTTCCACGACCATTGGAAAGGTTCCCTTATCGGCCACTCCGAC AACAATGAAGACGAGGACCTAGGCCATCTGCACGTCACCTACGCTAACAA CTACTGGTACAACGTGTACAGCCGTACACCCCTGATCCGGTTCGCCACAG TGCACATCATCAACAACTATTGGGACAGCCTGATCGACACGGGCGTGAAC TGCCGTATGGATGCACAGGTGCTGATCCAGTCCTCCGCGTTCCACAACTG CCCCGACAGAGCGATCTTCTTCGCCGACTCAGACTACACCGGGTATGCTG TCGTAGACGATGTTGACCTGGGCGGCTCGAGTAACTCGGTGCCCGAGGGA ACCCTGACGCCTAGCTCCTTGCCTTATGCGGCCATTACTGCGCTGGGATC TGGCCAGGTTGCAAGCGTGATTCCGGGTACAGCCGGACAGAAATTTCTAG AACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCAT CATCATCATCATTGA |
| Amino Acid Sequence (clone): | SMNSRDMRFTPLFLLAAVAIASPAPDLNARHELTRRQASESCPIGYCTQN GGTTGGAAGDTVTVTNLADLTEAAESDGPLTIIVSGSISGSAKIRVASDK TIFGESGSSITGIGFYIRRVSNVIMRNLKISKVDADNGDAIGIDASSNVW VDHCDLSGDLSGGKDDLDGLVDISHGAEWITVSNTYFHDHWKGSLIGHSD NNEDEDLGHLHVTYANNYWYNVYSRTPLIRFATVHIINNYWDSLIDTGVN CRMDAQVLIQSSAFHNCPDRAIFFADSDYTGYAVVDDVDLGGSSNSVPEG TLTPSSLPYAAITALGSGQVASVIPGTAGQKFLEQKLISEEDLNSAVDHH HHHH |
| Primers Used (Restriction sites): | GAAAGCACGTGATATGAGGTTCACCCCTCTCT (Pml I) TACAGTCTAGAAATTTCTGTCCGGCTGTAC (Xba I) |
| pH optimum: | 8.5 |
| Specific activity (Substrate): | pectic acid |
| T optimum: | 37 |
| ph Range Link: | Link |
| Temperature Link: | Link |
| Optimal Expression Medium: | BMMY |
| Description: | active on pectic acid, Ca2+ is cofactor, produces GalA3-6 with unsaturated GalA at the NR end |
| References: | not yet available |
pectate lyase