Enzyme Function:pectin lyase
Accession number: AN2331.2
GenBank ID: DQ490478
FGSC number: 10076
Average molecular weight
(clone, calculated):
43.195 kDa
AA length (clone): 407 aa
DNA length (clone): 1224 bp
DNA source: xylan cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGAAGACCACTTTCCTCGTTTCCCTGGCCAC CGCTGCCCTCAGCAGCACCGCCGCCGCCGTCTCCGTCAGCGGCTCGGCCG AGGGTTTCGCCAAGGGTGTGACCGGCGGTGGTAGTGCCACAGCCGTCTAC CCAGACACCATCGACGAGCTGGTCTCGTACCTGGGAGACGACGAGGCCCG CGTCATTGTCCTCTCTAAGACCTTCGACTTCACCGACAGTGAAGGCACGA CCACCGAAACCGGTTGCGCACCTTGGGGCACCGACGCGGCGTGCCAGGTC GCGATTAACCAGAATGACTGGTGCACAAACTACCAGCCTGACGCTCCCTC CGTCTCGGTGACCTACGACAATGCCGGAACCCTCGGTATCACCGTTAACT CGGACAAAACGCTTATCGGCGAGGGCTCCGCCGGTGTGATCAAGGGCAAG GGTCTGCGCCTTGTTAGCGGAACCAGCAATGTCATCATCCAAAACATCGC CATTACTGATATCAACCCCAAGTACGTCTGGGGAGGTGACGCCATCACTC TGAACGACGTGGATATGGTCTGGATCGACCACGTTACTACCGCTCGCATT GCTCGTCAGCACATCGTCCTCGGCACCGAAGCCGACAACCGCGTGACCAT CTCCAACTCGTTCATCAACGGTGAATCCGACTACTCGGCCACTTGCGATG GTTACCACTACTGGGGTATCTACCTCGACGGCTCCAGCGACATGGTCACC ATGAAGGGCAACTACATCTACCACACCAGCGGTCGTAGTCCCAAGGTCCA GGGTAACACTCTGCTGCACGCTGTCAACAACTACTGGCACGACAACTCTG ATCACGCCTTCGAGATCGGTGAGGGTGCCTACGTGCTCGCTGAAGGAAAC GTTTTCCAGAACATCCCCACCGTGGCCGAGGACCCCATTGAGGGTGAGCT CTTCGCTTCTCCCTCCGAATCTGCCAACGAGGTTTGCTCGACTTACCTTG GCCGTGTTTGCGAGCTCAACGGGTTCGGCAGCTCTGGCACCTTCAACCAG GCCGACACCGATTTCCTCAGCAAGTTCGAGGGCAAGAACATCGCATCCGC CGACTCCTACAGCAGCGTCGCTTCTAGTGTCGCCTCTTCTGCCGGTAACA CCCTTCTAGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTC GACCATCATCATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMKTTFLVSLATAALSSTAAAVSVSGSAEGFAKGVTGGGSATAVY PDTIDELVSYLGDDEARVIVLSKTFDFTDSEGTTTETGCAPWGTDAACQV AINQNDWCTNYQPDAPSVSVTYDNAGTLGITVNSDKTLIGEGSAGVIKGK GLRLVSGTSNVIIQNIAITDINPKYVWGGDAITLNDVDMVWIDHVTTARI ARQHIVLGTEADNRVTISNSFINGESDYSATCDGYHYWGIYLDGSSDMVT MKGNYIYHTSGRSPKVQGNTLLHAVNNYWHDNSDHAFEIGEGAYVLAEGN VFQNIPTVAEDPIEGELFASPSESANEVCSTYLGRVCELNGFGSSGTFNQ ADTDFLSKFEGKNIASADSYSSVASSVASSAGNTLLEQKLISEEDLNSAV DHHHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGAAGACCACTTTCCTCG (Pml I) TACAGTCTAGAAGGGTGTTACCGGCAGAA (Xba I)
pH optimum: 7.0
Specific activity (Substrate): citrus pectin
T optimum: n.d.
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: active on citrus pectin produces GalA oligomers with unsaturated methyl esterified GalA at the NR end
References: not yet available