Enzyme Function:pectate lyase
Accession number: AN3337.2
GenBank ID: DQ490486
FGSC number: 10087
Average molecular weight
(clone, calculated):
30.313 kDa
AA length (clone): 282 aa
DNA length (clone): 849 bp
DNA source: pectin cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGTACCAGCCTCTGCTTCTCCTTCCCCTCCT CCTAACTTCGGCTTTTGCCAACCCCCACGATCCCCACATTCACCACTCCC TGGAGAAGCGGGCATCCTTCCCCATACCTTCCTCGAAGGGCAGCGTGACC TTCTCTTCGCCCAAGACCATCAGCGGCACTTTTGATGGAGGCATGAAGAC CTATGGCCGTGGCGTCAAGTGTACCGGTCAAGACGAAGGTGGTGACGAGG ACGCCGTCTTCATTCTCAAGGACGGTGCCACTCTTAAGAACGCCATCATC GGCGCCGATCAAATTGAGGGTGTCCACTGTGAGGGCTCCTGCACTATCGA GAACGTCTGGTGGACGGATGTCTGTGAAGATGCCCTATCCCTCAAGGGCA GCGGCAGTGGCACCCACAAGATCATTGGTGGTGGTGCCCGCAATGCTGAC GACAAGGTCATCCAGCACAACTCTGGCGGCAAGGTGATCATCCAGGACTT CACTGTGCAGAACTTCGGTAAGCTCTACCGGGCCTGCGGCAACTGCAAGA AGCAGTTCAAGCGCACTGTGAAGATCAGCGGTGTCAAGGCTTCTAGCGGC AAGGCCCTTGTCGGCATCAACTCCAACTACGGTGATACCGCTTCCATCAA AGGCTGCGCGACCAGTGTTAAGGAGATTTGTGTTGAGTATGAGGGTACCA ACAACAACAGCAAGGAGCCTAAGAAGAAGTCTTCTGGTCCTAGCAGCTAC TGCAAATACAGTGAACCTCTGAGCAAGTGTCTAGAACAAAAACTCATCTC AGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMYQPLLLLPLLLTSAFANPHDPHIHHSLEKRASFPIPSSKGSVT FSSPKTISGTFDGGMKTYGRGVKCTGQDEGGDEDAVFILKDGATLKNAII GADQIEGVHCEGSCTIENVWWTDVCEDALSLKGSGSGTHKIIGGGARNAD DKVIQHNSGGKVIIQDFTVQNFGKLYRACGNCKKQFKRTVKISGVKASSG KALVGINSNYGDTASIKGCATSVKEICVEYEGTNNNSKEPKKKSSGPSSY CKYSEPLSKCLEQKLISEEDLNSAVDHHHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGTACCAGCCTCTGCTTCTC (Pml I) TACAGTCTAGACACTTGCTCAGAGGTTCACTG (Xba I)
pH optimum: 9.2
Specific activity (Substrate): pectic acid
T optimum: 37
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: active on pectic acid, Ca2+ is cofactor, produces GalA3-5 with unsaturated GalA at the NR end
References: not yet available