Enzyme Function:pectin methyl esterase
Accession number: AN3390.2
GenBank ID: DQ490489
FGSC number: 10090
Average molecular weight
(clone, calculated):
38.157 kDa
AA length (clone): 353 aa
DNA length (clone): 1062 bp
DNA source: xylan cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGCGCGTTCAATCATACTTGTCGCTCTTCAG CCTTGTTGGCGCAGCTCTCTGCGCGCCTCGTGAGCACTTCAAGCGCACTG CCAGAACGTCTGCTCCGGCCGGCTGTCTCACCGTTGGAGGAAGCGGGACC TACTCGACGATCGGCGCTGCGTTTGCAGCTTTGGGCTCATCCTCGTCTGA GGCCTGCATCTACATATCAGCCGGGACCTACAAGGAGCAATTGACCTTCC AATACGCTGGGCCGTTGACCCTCTATGGCGAAACCACGGACACGAGCAGT TACAAGAAGAACACCGTCACGATAACCCATACGATTTCCTCACCTGAAGC AGGGTCCCTTGTTGCGAGTGCGACTGTCAATGCGGCCATGGATAACTTTA CCATGTACAACATCAATGTTGTGAATGGGTACGGGAAGGGGGCTCAGGCT GTCGCACTGGCTGCCAGCGGAGAACGCCAGGGTTACTATGGCTGCCAATT CCTTGGGTATCAAGATACGCTGTACGCACGCGTGGGCGTGCAGTACTACT CCAACTGCTATATTGAGGGGGCCGTAGACTACATATTCGGCGACGCAAGC GCCTGGTTCGGCGAATGCGACATCGTCTCCAACGGTGCAGGCTACATCAC CGCCATGTCGCGCGAGACAGCCTCCGATCCGGCCTGGTATTGCTTCGACC ACTGCAATATCTACGGAAAATCGGGGCTGGACTTGACCGGCGATGTATAC CTCGGACGGCCGTGGCGCGTCCTCGCGCGGGTCATCTATCAGAACTCGGA GCTGAGTGATATCATCAACGCGGCTGGATGGACGACTATGGCAGAAGGAG CCACGCCACTGTACTACGAGATCGGGAATACGGGTGACGGGGCAGACACG TCCAAAAGGCTGTATCTTAGCGAGATCAGTGCGGCTGTCACCAAGGCTAC GGTGCTGGGGAGCGACTGGACGGACTGGCTTGACTGGAGCTATCTAGAAC AAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCAT CATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMRVQSYLSLFSLVGAALCAPREHFKRTARTSAPAGCLTVGGSGT YSTIGAAFAALGSSSSEACIYISAGTYKEQLTFQYAGPLTLYGETTDTSS YKKNTVTITHTISSPEAGSLVASATVNAAMDNFTMYNINVVNGYGKGAQA VALAASGERQGYYGCQFLGYQDTLYARVGVQYYSNCYIEGAVDYIFGDAS AWFGECDIVSNGAGYITAMSRETASDPAWYCFDHCNIYGKSGLDLTGDVY LGRPWRVLARVIYQNSELSDIINAAGWTTMAEGATPLYYEIGNTGDGADT SKRLYLSEISAAVTKATVLGSDWTDWLDWSYLEQKLISEEDLNSAVDHHH HHH
Primers Used (Restriction sites): GAAAGCACGTGATATGCGCGTTCAATCATACT (Pml I) TACAGTCTAGATAGCTCCAGTCAAGCCAGTC (Xba I)
pH optimum: 8.0
Specific activity (Substrate): citrus pectin
T optimum: 30
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: active on citrus pectin, random, essentially complete de-esterification
References: not yet available