Enzyme Function:beta-1,6-glucanase
Accession number: AN3777.2
GenBank ID: DQ490491
FGSC number: 10093
Average molecular weight
(clone, calculated): 		49.533 kDa
AA length (clone): 437 aa
DNA length (clone): 1314 bp
DNA source: pectin cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGAAGTTCATCCTCCCCCTCTTCACTTCTCT CCCTGTAGCCCTCGCCTGGCTCCCGGGCATCGACAAAGACATTTACTCCG CCGCAGGCACAAACATCTTCAATGTGACATCTGCCTCCAGCAAACGCTGG CTCCCGGCCTCGAAAAAGATCCGCGGCGTCAACCTTGGCAGCCACTTTGT CATCGAGCCGTGGATGGCCAGCATGGCATGGAGCAATATGGGCTGCAGTG GGCAACGGTCCGAGTTCGACTGCGTGATGGCTCTCGGGCAGGAGACTGCC GACCAGGCCTTTGCGGACCACTGGGGGTCCTGGATCACGCAGGACGATAT CAACCAGATGGTACAGTACGGGCTCAACACAATCCGCATCCCGGTAGGGT ACTGGCTCAAAGAGGATCTTGTCTACGCGGACTCGGAACACTTCCCAAAA GGAGGGATAGGGTATCTTGAGGACGTTTGCGGGTGGGCAAGTGATGCGGG CATGTATATCATCATAGATCTGCATGGGGCTCCTGGAGCGCAGCAGCCTA AGCAGCCATTTACGGGACAGTATGCCCCTAACCCTGGCTTCTACCAGGAC TACCAGTACGACCGGGCGCTGGAGTTCCTCGAGTGGATGACGACAAGCAT CCACCAAAATAACAAGTTCCGCAACGTCGGCATGCTTGAGATAGTCAACG AGCCTGTCCAGAACGCAGACCAGGCCTCCTCCATGATCAACAGCTACTAC CCAAGCGCGTTCACTCGCATCCGCAACACTGAGTCATCCCTTGGCATCAC CAGCAACAACTACCTGCACATCCAAATGATGAACGAGAAATGGGGCTCTG GCGATCCGACCCAATCCCTGACAGACAATTACTTCGCCGCGTACGACGAC CACCGCTATGTGAAGTGGGACTCCAGCGTCGCCGTGGATAAGGAAAGCTA TATCAGCGCCTCCTGTGTCGACGACCGTGGCGGCAACTGGCCGACCATCG TGGGCGAGTGGAGTCTCAGCGTTCCTGACAATGTCGAGCACACTGCAGAC TGGGAGCCATCGTCCAACACGGACTTTTACGCGAGGTGGTTTGCGGCGCA GGCGATTGCGTATGAAAAGCAGGAGGGCTGGGTGTTCTGGTCGTGGAAGG CGCAGCTGGGGGACTACAGGTGGTCGTATAAAGATGCGGTTGATGCTGGG GTTATTCCAAAGGATCTGGATAGTATCTATGACTACTCGCCCTGTCTAGA ACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATC ATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMKFILPLFTSLPVALAWLPGIDKDIYSAAGTNIFNVTSASSKRW LPASKKIRGVNLGSHFVIEPWMASMAWSNMGCSGQRSEFDCVMALGQETA DQAFADHWGSWITQDDINQMVQYGLNTIRIPVGYWLKEDLVYADSEHFPK GGIGYLEDVCGWASDAGMYIIIDLHGAPGAQQPKQPFTGQYAPNPGFYQD YQYDRALEFLEWMTTSIHQNNKFRNVGMLEIVNEPVQNADQASSMINSYY PSAFTRIRNTESSLGITSNNYLHIQMMNEKWGSGDPTQSLTDNYFAAYDD HRYVKWDSSVAVDKESYISASCVDDRGGNWPTIVGEWSLSVPDNVEHTAD WEPSSNTDFYARWFAAQAIAYEKQEGWVFWSWKAQLGDYRWSYKDAVDAG VIPKDLDSIYDYSPCLEQKLISEEDLNSAVDHHHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGAAGTTCATCCTCCCCC (Pml I) TACAGTCTAGACAGGGCGAGTAGTCATAGATA (Xba I)
pH optimum: n.d.
Specific activity (Substrate): n.d.
T optimum: n.d.
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: active on pustulan (beta-1,6-glucan)
References: not yet available