| Enzyme Function: | cellobiohydrolase |
| Accession number: | AN5282.2 |
| GenBank ID: | DQ490497 |
| FGSC number: | 10100 |
| Average molecular weight (clone, calculated): | 50.351 kDa |
| AA length (clone): | 483 aa |
| DNA length (clone): | 1452 bp |
| DNA source: | xylan cDNA |
| DNA Sequence (clone): | TCGATGAATTCACGTGATATGCACTACTCTGCTTCAGGTCTCGCGTTGGC ATTCTTGCTTCCTGCAATTCAGGCTCAGCAAACTCTCTATGGGCAATGTG GGGGATCTGGCTGGACCGGTGCTACGAGCTGTGTAGCTGGCGCAGCGTGT TCTACTCTGAACCAATGGTACGCTCAGTGCTTGCCAGCCGCAACCACCAC GTCTACTACCCTGACCACGACAACATCTTCAGTAACCACGACGAGCAATC CTGGCTCTACGACAACTACAAGCTCTGTTACTGTCACTGCCACTGCATCT GGAAACCCTTTCAGTGGCTACCAGCTCTACGTCAACCCTTACTACTCATC TGAGGTGCAAAGTATCGCCATTCCATCCCTCACTGGGACACTCTCCTCGC TTGCACCGGCAGCCACTGCTGCCGCCAAGACAAGAGATGTTGCCGCAAAG GTGCCCACAATGGCGACCTACCTCGCGGATATCCGGTCACAGAATGCTGC CGGAGCCAACCCTCCTATTGCAGGCCAATTTGTTGTTTATGACCTTCCGG ACCGTGACTGCGCCGCTCTGGCCAGTAATGGCGAGTTTGCCATTTCAGAT GGCGGGGTGCAGCATTATAAGGATTATATTGATTCCATCCGCGAAATTCT CGTGGAGTACTCAGACGTCCACGTAATCCTGGTTATTGAACCGGACAGTT TAGCTAATCTTGTCACAAACTTGAACGTTGCTAAATGTGCCAACGCCCAG AGCGCTTACCTGGAATGCACAAACTACGCGGTCACGCAGCTCAACCTTCC GAACGTGGCCATGTATCTTGACGCTGGACATGCCGGCTGGCTTGGCTGGC CAGCAAACCTCCAGCCCGCCGCAAACCTCTACGCAGGAGTCTACTCAGAC GCCGGGTCACCGGCCGCACTTCGTGGTCTTGCTACCAACGTCGCCAATTA CAACGCCTGGGCAATCGATACCTGCCCTTCATACACGCAAGGTAACTCCG TTTGTGACGAAAAGGACTACATCAACGCCCTCGCTCCCCTGCTTCGCGCT CAGGGCTTTGACGCTCACTTCATCACAGATACGGGCCGCAACGGGAAGCA GCCAACAGGCCAACAAGCCTGGGGCGACTGGTGCAACGTCATCGGCACGG GCTTCGGCGCGCGCCCATCCACGAACACAGGTGATTCCTTATTGGATGCT TTTGTCTGGGTTAAACCTGGTGGCGAGAGTGACGGGACTTCTGATACGTC CGCGGCGCGGTATGATGCGCATTGCGGGTATAGCGACGCGCTGCAGCCAG CGCCCGAGGCGGGAACTTGGTTTCAGGCATATTTCGTGCAGCTTTTGCAG AATGCGAACCCGTCTTTTCTAGAACAAAAACTCATCTCAGAAGAGGATCT GAATAGCGCCGTCGACCATCATCATCATCATCATTGA |
| Amino Acid Sequence (clone): | SMNSRDMHYSASGLALAFLLPAIQAQQTLYGQCGGSGWTGATSCVAGAAC STLNQWYAQCLPAATTTSTTLTTTTSSVTTTSNPGSTTTTSSVTVTATAS GNPFSGYQLYVNPYYSSEVQSIAIPSLTGTLSSLAPAATAAAKTRDVAAK VPTMATYLADIRSQNAAGANPPIAGQFVVYDLPDRDCAALASNGEFAISD GGVQHYKDYIDSIREILVEYSDVHVILVIEPDSLANLVTNLNVAKCANAQ SAYLECTNYAVTQLNLPNVAMYLDAGHAGWLGWPANLQPAANLYAGVYSD AGSPAALRGLATNVANYNAWAIDTCPSYTQGNSVCDEKDYINALAPLLRA QGFDAHFITDTGRNGKQPTGQQAWGDWCNVIGTGFGARPSTNTGDSLLDA FVWVKPGGESDGTSDTSAARYDAHCGYSDALQPAPEAGTWFQAYFVQLLQ NANPSFLEQKLISEEDLNSAVDHHHHHH |
| Primers Used (Restriction sites): | GAAAGCACGTGATATGCACTACTCTGCTTCAGGT (Pml I) TACAGTCTAGAAAAGACGGGTTCGCATTC (Xba I) |
| pH optimum: | 5.5 |
| Specific activity (Substrate): | CMC |
| T optimum: | 57 |
| ph Range Link: | Link |
| Temperature Link: | Link |
| Optimal Expression Medium: | BMMY |
| Description: | active on soluble CMC, active on cellooligos Glc3/Glc4/Glc5/Glc6 producing mainly Glc and Glc2, active on barley beta-glucan and lichenan, active on avicel 1 silent mutation in Pichia clone: nucleotide 1347 is A instead of G. |
| References: | not yet available |
cellobiohydrolase