Enzyme Function:endo-beta-1,4-galactanase
Accession number: AN5727.2
GenBank ID: DQ490498
FGSC number: 10102
Average molecular weight
(clone, calculated): 		41.870 kDa
AA length (clone): 378 aa
DNA length (clone): 1137 bp
DNA source: xylan cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGATTCTCTCCTCCCTCTTACCATTATCTCT GGTCACTCTCACCAGCGCCGCCCTGACCTACCGCGGCGCCGATATCTCCT CGCTCCTGATTGAAGAAGACTCCGGTGTCGCCTACAAGAATCTCAACGGT GAGACCCAAGCCTTCGAATTGATTCTAGCCAACAATGGAGTAAATTCGAT ACGGCAACGAATCTGGGTGAATCCAAGCGACGGATCTTACAACCTGGAGT ACAATCTGGAGCTGGCAAAGAGGGTACAGGATGCCGGCATGAGTGTCTAC TTGGACTTGCATTTGAGTGATACGTGGGCGGACCCGGGAGACCAGGCAAC CCCCTCTGGGTGGTCAACAACCGACATCGACACCCTGGCTTGGCAAGTGT ACAACTACACACTGGACGTGTGCAACACCTTCGCAGAGAACAATGTCGCC GTCGAGATTGTCTCCATCGGAAATGAAATCCGCAACGGCCTCCTTCACCC CTTAGGATCGACAGACCACTACGACAACATTGCGCGCCTCCTGCACTCGG GCGCCTGGGGCGTCAAGGACTCTTCGCTCTCCACGACGCCCAAGATCCTC TTCCACCTCGACAACGGGTGGGACTGGGATGCGCAGAAATACTTCTACGA CACCGTGCTTGCCACGGGTACACTGCTGTCTACTGATTTTGACCTTATTG GCGTATCGTACTACCCCTTCTACAATGCGGATGCAACGCTCTCCTCACTG AAAACGAGTTTGACGAACCTGAAGTCGAATTACGGCAAGAATGTGCTTGT TGTGGAGACGGATTGGCCGGTGCAGTGTAGCTCGCCTGAGTATGCATTTC CGTCGGATCTTAGTTCGATTCCTTTCAGCGCCGATGGGCAGGAGACTTTT CTGGGCAGGTTGGCGGACACGCTGGAGGATGTCGGTGGTGTGGGAATATA TTATTGGGAGCCTGGCTGGGTGGACAATGCGGGGCTGGGATCGAGCTGCG AAGATAACCTGATGGTTGACTGGAGGGACAGGACTGTGAGGGAAAGTATA AGTGTTTTTGGTGATCTTCTAGAACAAAAACTCATCTCAGAAGAGGATCT GAATAGCGCCGTCGACCATCATCATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMILSSLLPLSLVTLTSAALTYRGADISSLLIEEDSGVAYKNLNG ETQAFELILANNGVNSIRQRIWVNPSDGSYNLEYNLELAKRVQDAGMSVY LDLHLSDTWADPGDQATPSGWSTTDIDTLAWQVYNYTLDVCNTFAENNVA VEIVSIGNEIRNGLLHPLGSTDHYDNIARLLHSGAWGVKDSSLSTTPKIL FHLDNGWDWDAQKYFYDTVLATGTLLSTDFDLIGVSYYPFYNADATLSSL KTSLTNLKSNYGKNVLVVETDWPVQCSSPEYAFPSDLSSIPFSADGQETF LGRLADTLEDVGGVGIYYWEPGWVDNAGLGSSCEDNLMVDWRDRTVRESI SVFGDLLEQKLISEEDLNSAVDHHHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGATTCTCTCCTCCCTCTTA (Pml I) TACAGTCTAGAAGATCACCAAAAACACTTATACTT (Xba I)
pH optimum: n.d.
Specific activity (Substrate): n.d.
T optimum: n.d.
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: MMY
Description: active on potato pectic galactan, major products Gal and Gal2
References:
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