Enzyme Function:rhamnogalacturonan lyase
Accession number: AN7135.2
GenBank ID: DQ490504
FGSC number: 10108
Average molecular weight
(clone, calculated): 		59.689 kDa
AA length (clone): 555 aa
DNA length (clone): 1668 bp
DNA source: pectin cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGTTGTCCAAGACATTCCTTCTTTCCTCTGC CGTCTTGTGGGCTAGGGTGGCTAATGCGGCCTTTGGCATTACCGACAACG GCAGTTCCTACACGATCGACGCCAACTCTCCCAACCCGCTGAAATTCACT GTCAATAAGTCCAGCTGCGACATCACATCAATTGTTTACTATGGATCCGA GTTTCAGTATTCCGGAAAAGGGAGTCATATTGGCTCCGGTCTCGGCTCGG CCACAGTCTCTGCGACTCAGAGCGGCGACTACATTAAGGTTACTTGCGAC ACATCAAGCTTGACGCACTATTTCGTTGTGCACAATGGTGACCCCATCAT CCATATGGCGACCTATATTACTGCAGAGCCTGATATTGGTGAGCTGCGGT TTATTGCTCGACTTAACTCAAACCTCCTGCCGAATGAAGAGCCATTTGGG GATGTATCTACGACATCCGGCGGGTCAGCTATCGAGGGCTCCGATGTATT CCTGGTCAACGGCGAGACCCGTAGCAAATTCTACTCCAGCGAACGGTTTA TCGATGACCACAGGCACTGCATTTCGGGAAGCGCACACCGTGTCTGCATG ATCTTGAACCAATACGAGTCTTCCTCTGGTGGTCCTTTCTTCCGCGACAT CAACTCCAATAACGGCGGTGATTACAATGCCCTCTATTGGTACATGAACT CCGGCCACGTCCAAACCGAATCATTCCGTACGGGCCTCCACGGTCCCTAC TCGATGTACTTCAGTCGAAGCGGAACCCCCAGCACCAACATCGACACATC CTTCTTCGCCAGCCTTGGTATTAAGGGTTATGTTGCTGCCAACGGACGTG GAACTGTCACCGGCAAAGCTTCCGGCGCAGACTCGAGCATGGACTGGGTG GTTCACTGGTACAACAATGATGCCCAGTACTGGACCTACACAGCGTCCGA CGGCAGTTTCACCTCTCCTGCCATGAAACCCGGCACGTACACGATGAAAT ACTATCAAGGCGAATTCCCAGTGGCGGAAACCACCGTGACTGTATCGGCC GGATCATCGACCACGAAAAACATCTCCGGCTCGGTCAAAACAGGCACGAC GATCTTCAAAATTGGAGAATGGGATGGCCAACCAACTGGCTTCCGCAACG CAGATAAACAACTCCGCATGCACCCCTCCGACTCCCGGATGGACTCCTGG TCTTCCACCTACACAGTTGGCTCTTCTTCGCTCTCTGACTTCCCCATGGC TGTCTTCAAGAGCGTCAATAACCCCGTGACGATCAAGTTCACCGCTACTT CTGCACAGACAGGTGCTGCAACTCTCCGCATCGGCACAACCCTCTCCTTC GCTGGTGGTCGGCCGCAGGCAACGATCAACAGCTATACCGGCCCGGCTCC CTCAGCGCCTACTAATCTCAACTCCCGAGGTGTCACGCGAGGTGCTTACC GTGGGCTTGGGGAGGTGTATGATGTTAGCGTGCCCGCTGGAACAATTGTT ACGGGCGAGAATACGATCACTATAAGCGTTATTTCCGGAAGCTCAGGTGA CGCCTTCTTGAGCCCTAACGTGGTTTTTGATTGCATTGAACTTTTCCATC TAGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCAT CATCATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMLSKTFLLSSAVLWARVANAAFGITDNGSSYTIDANSPNPLKFT VNKSSCDITSIVYYGSEFQYSGKGSHIGSGLGSATVSATQSGDYIKVTCD TSSLTHYFVVHNGDPIIHMATYITAEPDIGELRFIARLNSNLLPNEEPFG DVSTTSGGSAIEGSDVFLVNGETRSKFYSSERFIDDHRHCISGSAHRVCM ILNQYESSSGGPFFRDINSNNGGDYNALYWYMNSGHVQTESFRTGLHGPY SMYFSRSGTPSTNIDTSFFASLGIKGYVAANGRGTVTGKASGADSSMDWV VHWYNNDAQYWTYTASDGSFTSPAMKPGTYTMKYYQGEFPVAETTVTVSA GSSTTKNISGSVKTGTTIFKIGEWDGQPTGFRNADKQLRMHPSDSRMDSW SSTYTVGSSSLSDFPMAVFKSVNNPVTIKFTATSAQTGAATLRIGTTLSF AGGRPQATINSYTGPAPSAPTNLNSRGVTRGAYRGLGEVYDVSVPAGTIV TGENTITISVISGSSGDAFLSPNVVFDCIELFHLEQKLISEEDLNSAVDH HHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGTTGTCCAAGACATTCCTT (Pml I) TACAGTCTAGATGGAAAAGTTCAATGCAATC (Xba I)
pH optimum: n.d.
Specific activity (Substrate): n.d.
T optimum: n.d.
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: MM
Description: active on linseed RG produces (GR)n oligomers with unsaturated GalA at the NR end 1 silent mutation in Pichia clone: nucleotide 156 is T instead of C
References: not yet available