| Enzyme Function: | endo-polygalacturonase |
| Accession number: | AN8327.2 |
| GenBank ID: | DQ490517 |
| FGSC number: | 10124 |
| Average molecular weight (clone, calculated): | 42.658 kDa |
| AA length (clone): | 408 aa |
| DNA length (clone): | 1227 bp |
| DNA source: | xylan cDNA |
| DNA Sequence (clone): | TCGATGAATTCACGTGATATGTTCTACGCTCTTGGACCTCTTGCCCTCTT CGCTTTCGCGACTGAGGTGATGGCCACCCCTGTGGCCTATCCAATGACCA CCGCGTCTCCAACTCTGGCCAAGCGGGACTCTTGCACCTTCTCAGGCTCG GACGGTGCTGCTTCTGCTAGCAGGTCGCAGACCGACTGCGCGACTATCAC TCTGTCCGACATCACCGTTCCATCGGGCACTACCCTCGACCTGAGCGACC TCGAGGACGATACCACTGTCATCTTCGAGGGCACCACCTCCTGGGAGTAC GAGGAGTGGGATGGACCGCTGCTCCAGATCAAGGGCAACGGCATCACTAT CAAGGGCGCCGATGGAGCCAAGCTGAACCCCGACGGATCCCGCTGGTGGG ATGGTGAGGGCTCCAACGGCGGCGTTACCAAGCCCAAGTTTTTCTACGCC CACGATCTGACCGACTCGACCATCCAGAACCTCTACATTGAGAACACCCC CGTCCAGGCCGTCAGCATCAACGGTTGCGATGGGCTGACCATCACCGACA TGACAATTGACAACTCCGCCGGTGACGATGCGGGTGGTCACAACACAGAC GGCTTCGATATCGGCGAGAGCTCCAACGTGGTCATTACCGGCGCCAAGGT CTACAACCAGGACGACTGTGTTGCTGTTAATTCCGGCACTTCCATCACCT TCAGCGGCGGTACCTGCTCTGGTGGACACGGTCTGTCGATCGGCAGCGTC GGTGGACGCGACGACAACACGGTCGACACCGTCACCTTTAAGGACTCAAC CGTCTCCAACTCTGTCAACGGCATTCGCATCAAGGCCAAGTCCGGCGAGA CCGGTGAGATCAAGGGCGTCACCTACTCTGGTATCAGCCTCGAGTCCATA TCCGATTATGGTATCCTCATTGAACAAAACTATGACGGCGGTGATCTCGA CGGCGAGGTGACCTCGGGCATCCCCATTACCGACCTCACCATTGAGAACA TCTCCGGCTCCGGCGCCGTTGACTCAGATGGATACAACATTGTCATCGTC TGCGGTGACGACGCCTGCTCCAACTGGACCTGGTCCGACGTCGAGGTTAC CGGTGGTGAGGATTACGGCAGCTGTGAGAACGTCCCCAGTGTTGCATCTT GCAGCACTCTAGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCC GTCGACCATCATCATCATCATCATTGA |
| Amino Acid Sequence (clone): | SMNSRDMFYALGPLALFAFATEVMATPVAYPMTTASPTLAKRDSCTFSGS DGAASASRSQTDCATITLSDITVPSGTTLDLSDLEDDTTVIFEGTTSWEY EEWDGPLLQIKGNGITIKGADGAKLNPDGSRWWDGEGSNGGVTKPKFFYA HDLTDSTIQNLYIENTPVQAVSINGCDGLTITDMTIDNSAGDDAGGHNTD GFDIGESSNVVITGAKVYNQDDCVAVNSGTSITFSGGTCSGGHGLSIGSV GGRDDNTVDTVTFKDSTVSNSVNGIRIKAKSGETGEIKGVTYSGISLESI SDYGILIEQNYDGGDLDGEVTSGIPITDLTIENISGSGAVDSDGYNIVIV CGDDACSNWTWSDVEVTGGEDYGSCENVPSVASCSTLEQKLISEEDLNSA VDHHHHHH |
| Primers Used (Restriction sites): | GAAAGCACGTGATATGTTCTACGCTCTTGGACC (Pml I) TACAGTCTAGAGTGCTGCAAGATGCAACAC (Xba I) |
| pH optimum: | 4.8 |
| Specific activity (Substrate): | pectic acid |
| T optimum: | 48 |
| ph Range Link: | Link |
| Temperature Link: | Link |
| Optimal Expression Medium: | BMMY |
| Description: | active on pectic acid, less active on citrus pectin, produces GalA1-3 |
| References: | not yet available |
endo-polygalacturonase