Enzyme Function:exo-polygalacturonase
Accession number: AN9045.2
GenBank ID: DQ490521
FGSC number: 10129
Average molecular weight
(clone, calculated): 		52.460 kDa
AA length (clone): 477 aa
DNA length (clone): 1434 bp
DNA source: gum arabic cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGGGTTTCAAAAGAACAATTGGCCTCTTGTT AGGCATCCTGCTGGCCCTCGACCAAGTCTCAGTGCTGGCTCAGCCCGGTC GTCCTACCTTTGCAAAACGTCCAGACATCCAACCGAATCCCATCCAGCCG TACAAGGCAATCCCCCTCCACTCTCGGCGTAATTCCCACAAGGTATGCTA TGTCAAGCCTAGCCTCAACGGGGGTGACGATGCCGGGAGAGTCGAAGCTG CACTGCGACGGTGCAACAACGGCGGGACAATTGTGCTCGACAAGGAGTAC TCCATCTGCACTCCTCTTGATCTACGCTTCTTAAAGCATGTGGACGTCGC CCTGACCGGAAAGGTCGAGTTCTGCCCCGAGCTGGAATTCTGGCAGCAGA ACGTGTTTCAATTCCATTTCCAGAATGCCTCCTCCTGGTGGGTTTGGGGC GGCGAGGATATTCACCTCTACGGCGCGGGCACCGGTGTCATCCATGGGAA CGGCCAGCCCTGGTGGGACGCGGCCGCAGGCAATTCGAGCGTTAGACGCC CGTTGCTATTTATTACAGATGGTTGGCACGGCGGTTCCATTACCGGCCTG AAGCTGCGGCAGTCACCCAACTGGCACAACTTCATCGCCAACAGCTCCGA CCTTCTCATCTCTGACATGGATATCTTCTCGCGCTCATCGTCGGAGGCCT GGGCATCGAACTTGGACGGCTGGGACACGTTCCGCTCAGACAACGTGGTT ATTCAGAACTCTGTCATCAATCACGATGACGACTGCGTCTCGTTCAAACC AAACAGCACCAATATTATCGTCCAGGGCTTGCACTGTAACGGCTCCCACG GGATCTCGGTTGGCTCGCTTGGAAACTACCCGTATCAGTACGATATCGTC TCAGACCTGTACATCTACAACAACACTATGGCCAACACCACCACGGCAGC ACGACTCAAGGTCTGGCCAGGCGCTGAGGCCGTCAAGAAAGGCAATCCGC CCTGGGTCGGTGGGGGAGGCAAGGGATACGTGCGCAACGTTACCTATGAC CTGATGATCAATGATAACAACGACCTCGCCATTCAAATTGACCAGTGCTA CGGGGCCATCAATGCGAGCGAGTGCTTGGATCACCCGTCCGGCGTCATTC TGACCAACGTACTCTTCAAGAATATGTGGGGTACCTCAAATGGTGCGAAT GATCCCGTTGCTGGCCAGCTTATCTGTGGTTCAGCGGATTCCTGCGACAA TATTCGAGCGGAGAATGTCACCCTGACGAACTCCAGTGGGCAGCCGTCGG AGTGGCAGTGTAGATACATGGATGAAGAGCTTCTGGATCTTGGTGGCGTC GGTTGCATCCCTGCTCTAGAACAAAAACTCATCTCAGAAGAGGATCTGAA TAGCGCCGTCGACCATCATCATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMGFKRTIGLLLGILLALDQVSVLAQPGRPTFAKRPDIQPNPIQP YKAIPLHSRRNSHKVCYVKPSLNGGDDAGRVEAALRRCNNGGTIVLDKEY SICTPLDLRFLKHVDVALTGKVEFCPELEFWQQNVFQFHFQNASSWWVWG GEDIHLYGAGTGVIHGNGQPWWDAAAGNSSVRRPLLFITDGWHGGSITGL KLRQSPNWHNFIANSSDLLISDMDIFSRSSSEAWASNLDGWDTFRSDNVV IQNSVINHDDDCVSFKPNSTNIIVQGLHCNGSHGISVGSLGNYPYQYDIV SDLYIYNNTMANTTTAARLKVWPGAEAVKKGNPPWVGGGGKGYVRNVTYD LMINDNNDLAIQIDQCYGAINASECLDHPSGVILTNVLFKNMWGTSNGAN DPVAGQLICGSADSCDNIRAENVTLTNSSGQPSEWQCRYMDEELLDLGGV GCIPALEQKLISEEDLNSAVDHHHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGGGTTTCAAAAGAACAATT (Pml I) TACAGTCTAGAGCAGGGATGCAACCGAC (Xba I)
pH optimum: n.d.
Specific activity (Substrate): n.d.
T optimum: n.d.
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: degrades end labeled GalA oligomers to labeled GalA4
References: not yet available