Glutamate Sensor Data
Variant
Recognition element
FRET Pair
Apo ratio*
Delta ratio
Delta ratio (%)
K
d
(
µ
M)
E. coli
Yeast
Mammalian
cells
Plants
Addgene pRSET-B
Addgene pcDNA
Addgene pDisplay
cytosol
plasma membrane
Cytosol
FLIPE-600n
E.coli
ECFP-Venus
2.0
1.7
-14
0.6
pRSETB
pDRf1GW
pcDNA3.1
pDisplay
CF203
Addgene pRSET-B link
AddGene pcDNA link
AddGene pDisplay link
FLIPE-10
µ
-1
E.coli
ECFP-Venus
1.6
0.7
-56
10
pRSETB
pDRf1GW
pcDNA3.1
pDisplay
CF203
Addgene pRSET-B link
AddGene pcDNA link
AddGene pDisplay link
FLIPE-10
µ
-2
E.coli
ECFP-Venus
5.8
1.7
-71
10
pRSETB
CF203
FLIPE-100
µ
E.coli
ECFP-Venus
1.4
1.2
-13
100
pRSETB
pDRf1GW
pcDNA3.1
pDisplay
Addgene pRSET-B link
AddGene pcDNA link
AddGene pDisplay link
FLIPE-1m
E.coli
ECFP-Venus
1.3
1.2
-10
1000
pRSETB
pDRf1GW
pcDNA3.1
pDisplay
CF203
Addgene pRSET-B link
AddGene pcDNA link
AddGene pDisplay link
FLIPE-1m-2
E.coli
ECFP-Venus
2.0
1.5
-25
1000
pRSETB
FLII
81
E-1
µ
E.coli
ECFP-Venus
2.0
1.0
-50
1
pRSETB
pDRf1GW
pcDNA3.1
pDisplay
CF203
FLII
81
E-10
µ
E.coli
ECFP-Venus
1.6
0.8
-52
10
pRSETB
pDRf1GW
pcDNA3.1
pDisplay
CF203
FLII
81
E-1m
E.coli
ECFP-Venus
2.0
1.0
-50
1000
pRSETB
pDRf1GW
pcDNA3.1
pDisplay
CF203
